| 论文编号: |
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| 第一作者所在部门: |
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| 中文论文题目: |
Structural Studies Reveal Unique Non-canonical Regulators of G Protein Signaling Homology (RH) Domains in Sorting Nexins |
| 英文论文题目: |
Structural Studies Reveal Unique Non-canonical Regulators of G Protein Signaling Homology (RH) Domains in Sorting Nexins |
| 作者: |
Yulong Zhang,Rui Chen,Yan Dong,Jiabin Zhu, Kai Su,Jinsong Liu and Jinxin Xu |
| 论文出处: |
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| 刊物名称: |
JOURNAL OF MOLECULAR BIOLOGY |
| 年: |
2022 |
| 卷: |
434 |
| 期: |
21 |
| 页: |
167823 |
| 联系作者: |
Jinsong Liu |
| 收录类别: |
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| 影响因子: |
6.151 |
| 摘要: |
As a subgroup of sorting nexins (SNXs) that contain regulator of G protein signaling homology (RH) domain, SNX-RH proteins, including SNX13, SNX14 and SNX25, were proposed to play bifunctional roles in protein sorting and GPCR signaling regulation. However, mechanistic details of SNX-RH proteins functioning via RH domain remain to be illustrated. Here, we delineate crystal structures of the RH domains of SNX13 and SNX25, revealing a homodimer of SNX13 RH domain mediated by unique extended α4 and α5 helices, and a thiol modulated homodimer of SNX25-RH triggered by a unique cysteine on α6 helix. Further studies showed that RH domains of SNX-RH do not possess binding capacity toward Gα subunits, owing to the lack of critical residues for interaction. Thus, this study identifies a group of novel non-canonical RH domains that can act as a dimerization module in sorting nexins, which provides structural basis for mechanism studies on SNX-RH protein functions. |
| 英文摘要: |
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